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KMID : 0380020050200060458
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 6 p.458 ~ p.463
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Continuous Hydrogen Production by Heterotrophic Growth of Citrobacter amalonaticus Y19 in Trickle Bed Reactor
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Park Ji-Young
Lee Tae-Ho
Oh You-Kwan
Kim Jun-Rae
Seol Eun-Hee
Jung Gyoo-Yeol
Kim Mi-Sun
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Abstract
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[ ] from CO and water was continuously produced in a trickle bed reactor(TBR) using Citrobacter amalonaticus Y19. When the strain C. was cultivated in a stirred-tank reactor under a chemoheterotrophic and aerobic condition, the high final cell concentration of 13 g/L was obtained at 10 hr. When the culture was switched to an anaerobic condition with the continuous supply of gaseous CO, CO-dependent hydrogenase was fully induced and its hydrogen production activity approached 16 mmol/g cell/hr in 60 hr. The fully induced C. amalonaticus Y19 cells were circulated through a TBR packed with polyurethane foam, and the TBR was operated for more than 20 days for production. As gas retention time decreased or inlet CO partial pressure increased, production rate increased but the conversion from CO to decreased. The maximum production rate obtained was 16 mmol/L/hr at the gas retention time of 25 min and the CO inlet partial pressure of 0.4 atm. The high production rate was attributed to the high cell density in the liquid phase circulating the TBR as well as the high surface area of polyurethane foam used as packing material of the TBR.
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KEYWORD
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Citrobacter amalonaticus Y19, carbon monoxide, continuous H₂ production, trickle bed reactor
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